Last data update: 24 January 2024 16:39 CET
Host name: Escherichia coli K12 WG1289 (LMBP 12438)
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| Species: | Escherichia coli |
| Group: | K12 |
| Strain designation: | WG1289 |
| Accession number: | LMBP 12438 |
| Depositor: | This host strain was deposited by Dr D.E. Culham1 and Prof. Em. Dr J.M. Wood1. 1 Department of Molecular and Cellular Biology, University of Guelph, Guelph, Canada |
| Containment level: | This strain has been assigned the containment level Class 1 following the European Directive 2009/41/EC on the contained use of genetically modified organisms, and its updates (see also the Belgian risk group classification). |
| Medium: | LB-Lennox |
| Selection marker: | - |
| Temperature: | 37°C |
| Genotype: | ΔproP837::FRT ΔproW859::FRT ΔbetT856::FRT ΔotsA847::FRT ΔyehW854::FRT |
| Phenotype: | - |
| Properties: | - |
| Further information: | Gene yehW was deleted from E. coli WG1246 as described by Datsenko and Wanner (2000). The Keio collection isolate JW2116-2 served as donor of ΔyehW754::kan yielding strain WG1289 (ΔyehW854::FRT). WG1289 as a WG1246 derivative cannot synthesize trehalose (ΔotsA) or take up osmoprotectants via transporters BetT, ProP or ProU. It lacks the integral membrane subunit ProW of the ProU system. WG1246 is a derivative of MG1655. |
| Original reference: | Lang et al., Biochemistry 54 (2015), 5735-5747 [PMID: 26325238] [DOI: 10.1021/acs.biochem.5b00274] |
| Related reference: | Murdock et al., Appl. Environ. Microbiol. 80 (2014), 5366−5378 [PMID: 24951793] [DOI: 10.1128/AEM.01138-14] Datsenko and Wanner, Proc. Natl. Acad. Sci. U.S.A. 97 (2000), 6640−6645 [PMID: 10829079] [DOI: 10.1073/pnas.120163297] |
| Restricted use: | - BCCM MTA - The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department. |
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Refer in your Materials and Methods: |
Escherichia coli K12 WG1289 (LMBP 12438) is available at BCCM/GeneCorner. It was deposited by Dr D.E. Culham and Prof. Em. Dr J.M. Wood and was published in Lang et al., 2015. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.